John D. Gearhart, Ph.D. Professor of Gynecology and Obstetrics, Physiology, Comparative Medicine, and Population Dynamics Director of Stem Cell Biology Johns Hopkins University School of Medicine Room 779, Broadway Research Building Baltimore, MD 21205 Phone: (410)-614-3444, (410)-614-3976 Fax: (410)-955-7427 Email: gearhart@jhmi.edu

Research

Over the past several years, the major research effort in our laboratory has been the isolation and characterization of human embryonic stem cells. Current research deals with the use of these cells in deriving differentiating cell types in culture and the transplantation of these cells to various mouse models of degenerative disorders and injuries. The human cells are also being investigated as vehicles for gene therapies.

Publications

Shamblott,MJ, Axelman, J., Wang, S., Bugg, EM, Littlefield, JW., Donovan, PJ., Blumenthal, PD., Huggins, GR., and Gearhart, JD., Derivation of pluripotent stem cells from cultured human primordial germ cells. Proc Natl Acad Sci USA 95:13726-13731, 1998.

Gearhart JD. New potential for human embryonic germ cells. Science 282:1061-1062, 1999.

Solter D and Gearhart JD. Putting stem cells to work. Science 283:1468-1470, 1999.

Shamblott MJ, Axelman J, Littlefield JW, Blumenthal PD, Huggins GR, Cui Y, Cheng L, Gearhart JD. Human embryonic germ cell derivatives express a broad range of developmentally distinct markers and proliferate extensively in vitro. Proc Natl Acad Sci U S A. 2001 Jan 2;98(1):113-8.

Donovan PJ, Gearhart JD. The end of the beginning for pluripotent stem cells. Nature 414, 92 - 97 (2001)

Moran TH, Capone GT, Knipp S, Davisson MT, Reeves RH, Gearhart JD. The effects of piracetam on cognitive performance in a mouse model of Down's syndrome. Physiol Behav 2002 Nov;77(2-3):403-9.

Shih IeM, Hsu MY, Oldt RJ 3rd, Herlyn M, Gearhart JD, Kurman RJ. The Role of E-cadherin in the Motility and Invasion of Implantation Site Intermediate Trophoblast. Placenta 2002 Nov;23(10):706-15.

Shamblott MJ, Bugg EM, Lawler AM, Gearhart JD. Craniofacial abnormalities resulting from targeted disruption of the murine Sim2 gene. Dev Dyn 2002 Aug;224(4):373-80.

Onyango P, Jiang S, Uejima H, Shamblott MJ, Gearhart JD, Cui H, Feinberg AP. Monoallelic expression and methylation of imprinted genes in human and mouse embryonic germ cell lineages. Proc Natl Acad Sci U S A 2002 Aug 6;99(16):10599-604.