clear
ICE Groups >    
clear
Vascular Biology Program
clear
Faculty
Jeff W. M. Bulte, Ph.D.

Jeff W. M. Bulte, Ph.D.

Professor of Radiology, Biomedical Engineering,
and Chemical & Biomolecular Engineering
Director, Cellular Imaging Section
Institute for Cell Engineering

Russell H. Morgan Department of Radiology and Radiological Science
Division of MR Research
217 Traylor Bldg, 720 Rutland Ave
Baltimore, MD 21205
Phone 410-614-2733
email jwmbulte@mri.jhu.edu

Research

The clinical development of novel immune and stem cell therapies calls for suitable methods that can follow the fate of cells non-invasively in humans at high resolution. The Bulte lab has pioneered methods to label cells magnetically (using tiny superparamagnetic iron oxide nanoparticles) in order to make them visible by MR imaging (Click here). Following years of extensive animal research, this technology has recently been introduced in the clinic for dendritic cell cancer vaccines. MRI cell tracking is further pursued in animal models of dysmyelination, multiple sclerosis (with Ben-Hur) , brain tumors, spinal cord (Click here) stroke (Click here), and myocardial infarct (with Kraitchman). Novel reporter genes are also being developed that can provide contrast on MRI scans. Artificial proteins are being designed, cloned, and expressed in mammalian cells that contain specific proton exchangable groups of which the proton signal can be manipulated (with van Zijl). A new direction is not directly labeling cells, but instead labeling semi-permeable microcapsules (Click here) that offer immunoprotection of cellular therapeutics (Click here) The current focus is on encapsulated pancreatic beta cells that can cure diabetes in mice and are being used in a large animal (swine) model. While the lab is doing basic bench-type research, there is a strong interaction with the clinical interventional radiology (Arepally) and oncology (Levitsky) groups in order to bring the methodologies into the clinic.

Lab Members

Mike McMahon, Ph.D.

Mike McMahon, Ph.D.,
Assistant Professor,
mcmahon@mri.jhu.edu

Assaf Gilad, Ph.D.

Assaf Gilad, Ph.D.,
Assistant Professor,
assaf@mri.jhu.edu

Piotr Walczak, M.D.

Piotr Walczak, M.D.,
Assistant Professor,
pwalczak@mri.jhu.edu

Dian Arifin

Dian Arifin, Ph.D.
postdoctoral fellow,
darifin@mri.jhu.edu

Amnon Bar-Shir

Amnon Bar-Shir, Ph.D.
postdoctoral fellow
abarshi2@jhmi.edu

Heechul Kim

Heechul Kim, Ph.D.
postdoctoral fellow,
heechul@mri.jhu.edu

Guanshu liu

Guanshu Liu, Ph.D.
postdoctoral fellow,
guanshu@mri.jhu.edu

Yajie Liang

Yajie Liang, Ph.D.
postdoctoral fellow
yliang@mri.jhu.edu

Nidhi Rumpal

Nidhi Rumpal, Ph.D.
postdoctoral fellow
nrumpal1@jhmi.edu

Lisa Song

Lisa Song, Ph.D.
postdoctoral fellow
lisasonger@gmail.com

Kannie Chang

Kannie Chang, Ph.D.
visiting postdoctoral fellow,
kwychan@hkucc.hku.hk

Segun Bernard

Segun Bernard, B.S.
Technician,
segun@mri.jhu.edu

Chulani Galpoththawela

Chulani Galpoththawela, B.S.
Technician
cgalpot2@jhu.edu

Michael Gorelik Michael Gorelik, B.S.
Technician
mgoreli3@jhmi.edu
Anna Munsey

Anna Munsey, B.S.
Technician
amunsey1@jhmi.edu

Stacey Cromer Berman

Stacey Cromer Berman, B.S.
Ph.D. student,
StaceyCromerBerman@jhmi.edu

Inema Orukari Inema Orukari, B.S.
PREP Student
inema@jhmi.edu
   

Publications

A.A. Gilad, P.T. Winnard Jr., P.C.M. van Zijl, J.W.M. Bulte. MR reporter genes: Promises and pitfalls. NMR Biomed. 20, 275-290 (2007).

A.A. Gilad, M.T. McMahon, P. Walczak, P.T. Winnard Jr., V. Raman, H.W.M. van Laarhoven C.M. Skoglund, J.W.M. Bulte, P.C.M. van Zijl. Artificial reporter gene providing MRI contrast based on proton exchange. Nat. Biotechnol. 25, 217-219 (2007). [Pubmed]

B.P. Barnett, A. Arepally, P.V. Karmarkar, D. Qian, W.D. Gilson, P. Walczak, V. Howland, L. Lawler, C. Lauzon, M. Stuber, D.L. Kraitchman, J.W.M. Bulte. Magnetic resonance-guided, real-time targeted delivery and imaging of magnetocapsules immunoprotecting pancreatic islet cells. Nat. Med. 13, 986-991 (2007). [PubMed]

P. Walczak, J. Zhang, A.A. Gilad, D.A. Kedziorek, J. Ruiz-Cabello, R.G. Young, M.F. Pittenger, P.C.M. van Zijl, J. Huang, J.W.M. Bulte. Dual-modality monitoring of intraarterial mesenchymal stem cell delivery and brain engraftment following transient ischemia. Stroke 39, 1569-1574 (2008). [PubMed]

A.A. Gilad, P. Walczak, M.T. McMahon, H.B. Na, J.H. Lee, K. An, T. Hyeon, P.C.M. van Zijl, J.W.M. Bulte. MR tracking of transplanted cells with positive contrast using manganese oxide nanoparticles. Magn. Reson. Med., Magn. Reson. Med. 60, 1-7 (2008). [PubMed]

J.F.A. Jansen, J.D. Gearhart, J.W.M. Bulte. Magnetic resonance spectroscopy identifies neural progenitor cells in the live human brain. Science 321, 640 (2008). [PubMed]

J. Ruiz-Cabello, P. Walczak, D.A. Kedziorek, V.P. Chacko, A. H. Schmieder, S.A. Wickline, G.M. Lanza, J.W.M. Bulte. In vivo "hot spot" MR imaging of neural stem cells using fluorinated nanoparticles. Magn. Reson. Med. 60, 1506-1511
(2008). [PubMed]

C.M. Long, H.W.M. van Laarhoven, J.W.M Bulte, H.I. Levitsky. Magnetovaccination as a novel method to assess and quantify dendritic cell tumor antigen capture and delivery to lymph nodes. Cancer Res. 69, 3180-3187 (2009). [PubMed]

A.A. Gilad, H.W.M. van Laarhoven, M.T. McMahon, P. Walczak, A. Heerschap, M. Neeman, P.C.M. van Zijl, J.W.M. Bulte. Feasibility of concurrent dual contrast enhancement using CEST contrast agents and superparamagnetic iron oxide particles. Magn. Reson. Med. 61, 970-974 (2009). [PubMed]

J.W.M. Bulte. In vivo MRI cell tracking: clinical studies. Amer. J. Roentgenol. 193, 314-325 (2009). [PubMed]


Copyright © 2006 Institute for Cell Engineering. All Rights Reserved. Site designed by Academic Web Pages.